期刊
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
卷 1, 期 3, 页码 143-148出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jgar.2013.04.003
关键词
Salmonella; CMY-2; Fitness
资金
- Comision Sectorial de Investigacion Cientifica (CSIC, Uruguay)
The objectives of this study were to investigate clinical isolates of Salmonella enterica serovar Typhimurium resistant to p-lactam antibiotics, to characterise their mechanisms of antibiotic resistance and to evaluate the possible biological cost of expressing resistance genes. Two oxyimino-cephalosporin-resistant Salmonella isolates obtained from children with diarrhoea were characterised. The occurrence of plasmid-encoded bla(CMY-2) genes was confirmed by molecular methods and conjugation assays; transcription levels were determined by quantitative real-time PCR (qRT-PCR). The genomic context of the [beta-lactamases, replicon type and addiction systems were analysed by PCR. Genomic relatedness of both isolates was studied by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) assays. Growth curves, motility and invasiveness assays in Caco-2 cells were performed to analyse the bacterial fitness of both isolates. Both isolates carried a bla(CMY-2)-like allele in an IncI plasmid and belonged to the same MLST sequence type (ST19); nevertheless, they showed extensive differences in their PFGE profiles and virulotypes. Isolate STM709 appeared to lack the Salmonella virulence plasmid and displayed less motility and invasiveness in cultured cells than isolate STM910. qRT-PCR showed that isolate STM709 had higher bla(CMY-2) mRNA levels compared with STM910. Altogether, the results suggest that a plasmid carrying blacmy-2 could be disseminating among different clones of S. Typhimurium. Different levels of bla(CMY-2) mRNA could have an effect on the fitness of this micro-organism, resulting in lower invasiveness and motility. (C) 2013 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.
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