PPARγ inhibits ovarian cancer cells proliferation through upregulation of miR-125b

miR-125b has essential roles in coordinating tumor proliferation, angiogenesis, invasiveness, metastasis and chemotherapy recurrence. In ovarian cancer miR-125b has been shown to be downregulated and acts as a tumor suppressor by targeting proto-oncogene BCL3. PPAR gamma, a multiple functional transcription factor, has been reported to have anti-tumor effects through inhibition of proliferation and induction of differentiation and apoptosis by targeting the tumor related genes. However, it is unclear whether miR-125b is regulated by PPAR gamma in ovarian cancer. In this study, we demonstrated that the miR-125b downregulated in ovarian cancer tissues and cell lines. Ligands-activated PPAR gamma suppressed proliferation of ovarian cancer cells and this PPAR gamma-induced growth inhibition is mediated by the upregulation of miR-125b. PPAR gamma promoted the expression of miR-125b by directly binding to the responsive element in miR-125b gene promoter region. Thus, our results suggest that PPAR gamma can induce growth suppression of ovarian cancer by upregulating miR-125b which inhibition of proto-oncogene BCL3. These findings will extend our understanding of the function of PPAR gamma in tumorigenesis and miR-125b may be a therapeutic intervention of ovarian cancer. (C) 2015 Elsevier Inc. All rights reserved.