期刊
FRONTIERS IN MOLECULAR NEUROSCIENCE
卷 5, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fnmol.2012.00063
关键词
M-channel; Kv7; KCNQ; excitability; XE991; retigabine
资金
- Medical Research Council [G0500194]
- Wellcome Trust [083858]
- Neurosearch (Ballerup, Denmark) via EU [LSHM-CT-2004-503038]
- MRC [G0500194] Funding Source: UKRI
M-channels carry slowly activating potassium currents that regulate excitability in a variety of central and peripheral neurons. Functional M-channels and their Kv7 channel correlates are expressed throughout the somatosensory nervous system where they may play an important role in controlling sensory nerve activity. Here we show that Kv7.2 immunoreactivity is expressed in the peripheral terminals of nociceptive primary afferents. Electrophysiological recordings from single afferents in vitro showed that block of M-channels by 3 mu M XE991 sensitized but not C-fibers to noxious heat stimulation and induced spontaneous, ongoing activity at 32 degrees C in many A delta-fibers. These observations were extended in vivo: intraplantar injection of XE991 selectively enhanced the response of deep dorsal horn (DH) neurons to peripheral mid-range mechanical and higher range thermal stimuli, consistent with a selective effect on A delta-fiber peripheral terminals. These results demonstrate an important physiological role of M-channels in controlling nociceptive A delta-fiber responses and provide a rationale for the nocifensive behaviors that arise following intraplantar injection of the M-channel blocker XE991.
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