期刊
FRONTIERS IN MOLECULAR NEUROSCIENCE
卷 4, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fnmol.2011.00013
关键词
glycine receptors; endocannabinoids; desensitization; patch-clamp; metaplasticity
资金
- European Union Seventh Framework: NEUROCYPRES [HEALT-F2-2008-202088]
- Marie Curie Fellowship
- FRM grant Nouvelle Equipe
Endocannabinoids are known as retrograde messengers, being released from the postsynaptic neuron and acting on specific presynaptic G-protein-coupled cannabinoid (CB) receptors to decrease neurotransmitter release. Also, at physiologically relevant concentrations cannabinoids can directly modulate the function of voltage-gated and receptor-operated ion channels. Using patch-clamp recording we analyzed the consequences of the direct action of an endocannabinoid, 2-arachidonoylglycerol (2-AG), on the functional properties of glycine receptor channels (GlyRs) and ionic currents in glycinergic synapses. At physiologically relevant concentrations (0.1-1 mu M), 2-AG directly affected the functions of recombinant homomeric alpha 1 H GlyR: it inhibited peak amplitude and dramatically enhanced desensitization. The action of 2-AG on GlyR-mediated currents developed rapidly, within similar to 300 ms. Addition of 1 mu M 2-AG strongly facilitated the depression of glycine-induced currents during repetitive (4-10 Hz) application of short (2 ms duration) pulses of glycine to outside-out patches. In brainstem slices from CB1 receptor knockout mice, 2-AG significantly decreased the extent of facilitation of synaptic currents in hypoglossal motoneurons during repetitive (10-20 Hz) stimulation. These observations suggest that endocannabinoids can modulate postsynaptic metaplasticity of glycinergic synaptic currents in a CB1 receptor-independent manner.
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