期刊
FEBS OPEN BIO
卷 4, 期 -, 页码 441-449出版社
WILEY
DOI: 10.1016/j.fob.2014.04.010
关键词
Small RNA; Nucleolus; Deep sequencing; miRNA expression profiling
资金
- National Institutes of Health [P30 CA006973]
- Johns Hopkins University start-up funds
- Johns Hopkins Deep Sequencing and Microarray Core Facility
- Sidney Kimmel Comprehensive Cancer Center Next Generation Sequencing Core
Micro RNAs (miRNA) are non-coding RNAs expressed in the cytoplasm as their mature, 21-22-nucleotide short forms. More recently, mature miRNAs have also been detected in the nucleus, raising the possibility that their spatial distribution may be more complex than anticipated. Here we undertook comprehensive systematic analyses of miRNA distribution in several subcellular compartments of human cancer cells. In particular, we focused on the potential presence of miRNAs in the nucleolus, which contains an abundance of small non-coding RNAs. We employed two miRNA expression array platforms and small RNA deep sequencing of small RNAs isolated from cells, nuclei, cytoplasm and the nucleoli. We developed an assay to compare RNAs of isolated nucleoli before and after denaturation and used Northern hybridization to verify the presence of miRNAs in the subcellular compartments. Consistently, we found more than 10 miRNAs associated with the nucleolar preparations. Several miRNAs had greater relative abundance in the nucleolus compared to the other compartments. The nucleolar presence of miRNAs was independent of Dicer and the main activity of the nucleolus, RNA polymerase I transcription, but was dependent on CRM1 previously associated with nucleolar trafficking of small nucleolar RNAs. These results highlight the complexity of miRNA spatial arrangement and regulation. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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