期刊
CHEMELECTROCHEM
卷 1, 期 11, 页码 1886-1894出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/celc.201402276
关键词
bioelectrochemistry; biofuel cells; cellobiose dehydrogenase; enzymatic electrodes; laccase
资金
- German Research Foundation (DFG) through the Ph.D. program Micro Energy Harvesting [GRK 1322]
- State Graduate Funding Program of Baden-Wurttemberg
Enzymatic electrodes have great potential for catalysing the direct conversion of chemical compounds into electricity with the use of redox enzymes. However, expensive and time-consuming enzyme purification and the frequent need to add mediators are considered as drawbacks of enzymatic electrodes. We report a biofuel cell at pH5, supplied with the unpurified enzymes laccase and cellobiose dehydrogenase (CDH) in crude culture supernatant, without the further addition of mediators. A maximum power output of 6.2 +/- 1.2Wcm(-2) was achieved by using supernatants containing laccase from Trametes versicolor (2.84UmL(-1)) and CDH (0.90UmL(-1)) from a recombinant yeast Yarrowia lipolytica YPC4. In comparison, the supply of purified enzymes (laccase: 2.40UmL(-1), CDH: 0.15UmL(-1)) in a buffer solution yielded only about a twofold higher power density. Our results demonstrate the feasibility of using unpurified laccase and CDH in an enzymatic biofuel cell, which can simplify its construction and operation.
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