期刊
BIOLOGY OPEN
卷 2, 期 11, 页码 1160-1170出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/bio.20136163
关键词
Polarity; ISP; Plasmodium
类别
资金
- MRC [G0900109, MR/K011782/1, G0900278, U117532067]
- EU [242095, PCIG10-GA-2011-30408]
- Wellcome Trust
- Agence Nationale de la Recherche
- BBSRC [BB/J01477X/1]
- EPSRC
- BBSRC [BB/D02014X/1, BB/J01477X/1] Funding Source: UKRI
- MRC [MC_U117532067, G0900278] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D02014X/1, BB/J01477X/1] Funding Source: researchfish
- Medical Research Council [G0900278, MC_U117532067] Funding Source: researchfish
The phylum Apicomplexa comprises over 5000 intracellular protozoan parasites, including Plasmodium and Toxoplasma, that are clinically important pathogens affecting humans and livestock. Malaria parasites belonging to the genus Plasmodium possess a pellicle comprised of a plasmalemma and inner membrane complex (IMC), which is implicated in parasite motility and invasion. Using live cell imaging and reverse genetics in the rodent malaria model P. berghei, we localise two unique IMC sub-compartment proteins (ISPs) and examine their role in defining apical polarity during zygote (ookinete) development. We show that these proteins localise to the anterior apical end of the parasite where IMC organisation is initiated, and are expressed at all developmental stages, especially those that are invasive. Both ISP proteins are N-myristoylated, phosphorylated and membrane-bound. Gene disruption studies suggest that ISP1 is likely essential for parasite development, whereas ISP3 is not. However, an absence of ISP3 alters the apical localisation of ISP1 in all invasive stages including ookinetes and sporozoites, suggesting a coordinated function for these proteins in the organisation of apical polarity in the parasite. (C) 2013. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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