期刊
BIOLOGY OPEN
卷 1, 期 10, 页码 977-982出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/bio.2012646
关键词
Hsp90 alpha; Spermatogonia; Spermatogenesis; Androgen receptor
类别
It is controversial whether a functional androgen receptor (AR) on germ cells, including spermatogonia, is essential for their development into sperm and, thus, initiation and maintenance of spermatogenesis. It was recently shown that many spermatocytes underwent apoptosis in the testes of Hsp90 alpha KO mice. We had generated Hsp90 alpha KO mice independently and confirmed this phenotype. However, the important question of whether Hsp90 alpha is required to maintain spermatogenesis in adult mice in which testicular maturation is already completed could not be addressed using these conventional KO mice. To answer this question, we generated a tamoxifen-inducible deletion mutant of Hsp90 alpha and found that conditional deletion of Hsp90 alpha in adult mice caused even more severe apoptosis in germ cells beyond the pachytene stage, leading to complete arrest of spermatogenesis and testicular atrophy. Importantly, immunohistochemical analysis revealed that AR expression in WT testis was more evident in spermatogonia than in spermatocytes, whereas its expression was aberrant and ectopic in Hsp90 alpha KO testis, raising the possibility that an AR abnormality in primordial germ cells is involved in spermatogenesis arrest in the Hsp90 alpha KO mice. Our results suggest that the AR, specifically chaperoned by Hsp90 alpha in spermatogonia, is critical for maintenance of established spermatogenesis and for survival of spermatocytes in adult testis, in addition to setting the first wave of spermatogenesis before puberty. (C) 2012. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0).
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