A Simple Precipitation Approach for Isolation and Enrichment of Sugarcane Streak Mosaic Virus

Sugarcane streak mosaic virus (SCSMV) is a more prevalent sugarcane infecting virus reducing the total sugar production of the world which necessitates rapid screening and detailed studies on SCSMV pathogenicity. A simple reliable, user friendly approach for purification and enrichment of SCSMV from infected sugarcane leaf tissue was developed as alternative to ultracentrifugation approach. The quality and fold enrichment of viral particle was confirmed by RT-PCR and sequence analysis of SCSMV coat protein region. This approach offers 20.03-fold increase in sensitivity for their detection and high viral: host genome ratio of 10(2) and 10(3) in infected leaf tissue and purified viral fraction, respectively.