期刊
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
卷 21, 期 1, 页码 81-88出版社
ELSEVIER
DOI: 10.1016/j.sjbs.2013.06.004
关键词
Solid state fermentation; Penicillium purpurogenum; Box-Behnken design; Corn cob; Corn bran
类别
资金
- National Research Center, Chemistry of natural and microbial products department (Egypt)
Phytase production by Penicillium purpurogenum GE1 isolated from soil around bean root nodules was investigated by solid state fermentation (SSF) using mixed substrates consisted of corn cob and corn bran. The SSF conditions were optimized by using one-variable-at-a-time strategy. The optimum conditions for phytase production were at 27 degrees C, pH 8 and 66% moisture content. The study of different carbon and nitrogen sources revealed that glucose and peptone registered the highest enzyme productivity (92 +/- 5.6 U/g ds, 125 +/- 4.9 U/g ds). Among different surfactants, maximum phytase productivity was observed with Tween 80 at 0.001 concentrations (170 +/- 4.2 U/g ds). A Box-Behnken design was employed to investigate the optimization of the most significant variables affecting the enzyme production. Maximal phytase production was detected after the addition of (g/5 g ds): 0.75 glucose, 0.375 peptone and 0, 01 tween 80. This result represented an improvement in phytase production of 2.6 folds when compared to that previously obtained using the basal medium under the same cultivation conditions. The generated model was found to be very adequate for phytase production (90% accuracy) as the experimental value was 444 +/- 3.5 U/g ds compared to 401 U/g ds for the predicted value. In brief, the production of phytase using corn cob and corn bran is a novel and cheap way for the production of this important enzyme and opens a new way for researchers to discover and explore this arena. (C) 2014 Production and hosting by Elsevier B.V. on behalf of King Saud University.
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