期刊
PATHOGENS AND DISEASE
卷 72, 期 1, 页码 74-77出版社
OXFORD UNIV PRESS
DOI: 10.1111/2049-632X.12184
关键词
bioluminescence; Pseudomonas aeruginosa; in vivo imaging; infection monitoring; transposon mutant; mouse model
资金
- Deutsche Forschungsgemeinschaft [IRTG 653, SFB 587]
- German Center for Lung Research
Non-invasive bioluminescence imaging allows the analysis of infectious diseases in small animal models. In this study, an acute airway infection of C3H/HeN mice with luxCDABE transformed Pseudomonas aeruginosaTBCF10839 and an isogenic transposon mutant was followed by optical imaging in vivo. Using the disease-causing dose of 2.0x10(6)CFU of the cystic fibrosis airway isolate TBCF10839, subtle luminescence of the lungs was inconsistently visible for the first hour after infection. Conversely, using a 100-fold higher dose of the strongly virulence-attenuated transposon mutant, the robust signal of bioluminescent bacteria increased over 24h. To monitor murine airway infections with P.aeruginosa in vivo by bioluminescence, one should select an attenuated mutant of a virulent strain or a wild type strain that naturally lacks virulence determinants and/or that has acquired a low virulence persister phenotype by patho-adaptive mutations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据