期刊
NANOSCALE RESEARCH LETTERS
卷 9, 期 -, 页码 -出版社
SPRINGEROPEN
DOI: 10.1186/1556-276X-9-291
关键词
EGCG; Nanoliposomes; Optimization; Response surface methodology; Stability; Cellular uptake
资金
- Zhejiang Provincial Engineering Laboratory of Quality Controlling Technology and Instrumentation for Marine Food
- Natural Science Foundation of Zhejiang Province [LY14C200012]
- Zhejiang Provincial Public Technology Application Research Project [2012C22052]
- General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China [201310120]
- Hangzhou Science and Technology Development Project [20130432B66, 20120232B72]
- 'Five-twelfth' National Science and Technology Support Program [2011BAK10B03]
The major component in green tea polyphenols, epigallocatechin-3-gallate (EGCG), has been demonstrated to prevent carcinogenesis. To improve the effectiveness of EGCG, liposomes were used as a carrier in this study. Reverse-phase evaporation method besides response surface methodology is a simple, rapid, and beneficial approach for liposome preparation and optimization. The optimal preparation conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 4.00, EGCG concentration of 4.88 mg/mL, Tween 80 concentration of 1.08 mg/mL, and rotary evaporation temperature of 34.51A degrees C. Under these conditions, the experimental encapsulation efficiency and size of EGCG nanoliposomes were 85.79% A +/- 1.65% and 180 nm A +/- 4 nm, which were close with the predicted value. The malondialdehyde value and the release test in vitro indicated that the prepared EGCG nanoliposomes were stable and suitable for more widespread application. Furthermore, compared with free EGCG, encapsulation of EGCG enhanced its inhibitory effect on tumor cell viability at higher concentrations.
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