期刊
NANOMATERIALS AND NANOTECHNOLOGY
卷 2, 期 -, 页码 -出版社
SAGE PUBLICATIONS LTD
DOI: 10.5772/53926
关键词
quantum dots; fluorescence; glutathione; glutathione S-transferase fusion protein
资金
- Innovation and Science Ministry [PN/PETRI/PR/2007-019]
- Junta de Andalucia [P08-CTS-04348]
Advances in the life sciences are now closely linked to the availability of new experimental tools that allow for the manipulation of biomolecules and the study of biological processes at the molecular level. In this context, we have optimized a synthesis process to obtain glutathione-capped fluorescent CdS nanoparticles to specifically detect Glutathione S-Transferase (GST) -tagged proteins. Using our method, based on five different heating steps, brightly fluorescent and biocompatible CdS quantum dots of different sizes can be obtained. QD optical behaviour has been evaluated studying both absorbance and fluorescence. For all the samples, the excitonic absorption onset clearly shows a blue shift at 512nm in comparison with that of bulk CdS, due to the quantum confinement effect. At increased average sizes of the nanocrystal, the emission fluorescent band shows a red shift, from 440nm to 540nm. Among different QD solutions, we demonstrate an expansion of the emission range up to similar to 100 nm, thus improving their features as biomarkers. Moreover we show that optimized glutathione-capped quantum dots can directly bind GST blotted onto polyvinylidene difluoride (PVDF) membranes, and thus are suitable for the direct detection of GST fusion proteins.
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