Correlation of Specific Amyloid-β Oligomers With Tau in Cerebrospinal Fluid From Cognitively Normal Older Adults

Importance: To improve the ability to develop treatments that prevent incipient Alzheimer disease (AD) from progressing to overt AD, it is important to understand the molecular basis of the earliest pathophysiological abnormalities and to determine how amyloid-beta (A beta) is involved very early in its pathogenesis. Objective: To investigate 2 specific A beta oligomers, A beta trimers and A beta*56, in human cerebrospinal fluid (CSF); evaluate the effects of aging and AD; and obtain support for the hypothesis that they may be pathogenic by determining their relationships to CSF tau. Design: A CSF sampling study. Settings: The University of Minnesota Medical School in Minneapolis, Minnesota, and the Salhgrenska University Hospital, Sweden. Participants: Forty-eight older adults with mild cognitive impairment or AD (impaired group); 49 age-matched cognitively intact control subjects (unimpaired group); and 10 younger, normal control subjects. Main Outcome Measures: Measurements of CSF A beta trimers, A beta*56, the 42-amino acid A beta isoform (A beta 1-42), total tau (T-tau), and phospho-tau 181 (p-tau(181)). The hypothesis being tested was formulated after data collection. Results: We observed that A beta trimers and A beta*56 levels increased with age; within the unimpaired group, they were elevated in subjects with T-tau/A beta 1-42 ratios greater than a cutoff that distinguished the unimpaired group from subjects with AD. In the unimpaired group, T-tau and p-tau181 were found to correlate strongly with A beta trimers and A beta*56 (r > 0.63), but not with A beta 1-42 (-0.10 < r < -0.01). The strong correlations were found to be attenuated in the impaired group. Conclusions and Relevance: In cognitively intact older adults, CSF A beta trimers and A beta*56 were elevated in individuals at risk for AD, and they showed stronger relationships with tau than did A beta 1-42, a surrogate for A beta fibril deposition. These findings suggest that prior to overt symptoms, 1 or both of the A beta oligomers, but not fibrillar A beta, is coupled to tau; however, this coupling is weakened or broken when AD advances to symptomatic stages. The uncoupling is interesting in light of the failure of experimental A beta therapies to improve mild cognitive impairment/AD, which has prompted a shift in the timing of A beta therapies to asymptomatic subjects. Knowing which A beta species to target in asymptomatic subjects may enhance the success of future treatments for AD.