期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 12, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12951-014-0031-y
关键词
Nanoparticle; Uptake; Nanotoxicity; Neurons; Potassium channels; Firing rate
资金
- NIH/NIGMS MARC U*STAR [GM07717]
- National Institute on Minority Health and Health Disparities RCMI from the National Institutes of Health [G12MD007591]
- NSF PREM DMR [0934218]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [934218] Funding Source: National Science Foundation
Background: With the increased use of nanoparticles in biomedical applications there is a growing need to understand the effects that nanoparticles may have on cell function. Identifying these effects and understanding the mechanism through which nanoparticles interfere with the normal functioning of a cell is necessary for any therapeutic or diagnostic application. The aim of this study is to evaluate if gold nanoparticles can affect the normal function of neurons, namely their activity and coding properties. Results: We synthesized star shaped gold nanoparticles of 180 nm average size. We applied the nanoparticles to acute mouse hippocampal slices while recording the action potentials from single neurons in the CA3 region. Our results show that CA3 hippocampal neurons increase their firing rate by 17% after the application of gold nanostars. The increase in excitability lasted for as much as 50 minutes after a transient 5 min application of the nanoparticles. Further analyses of the action potential shape and computational modeling suggest that nanoparticles block potassium channels responsible for the repolarization of the action potentials, thus allowing the cell to increase its firing rate. Conclusions: Our results show that gold nanoparticles can affect the coding properties of neurons by modifying their excitability.
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