期刊
JOURNAL OF NANOBIOTECHNOLOGY
卷 10, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1477-3155-10-25
关键词
Molecular MRI; Liposome; ICAM-1; Endothelium; Leukocyte; Shear stress
资金
- Dutch Technology Foundation STW, applied science division of NWO
- Technology Program of the Ministry of Economic Affairs [07952]
Background: The upregulation of intercellular adhesion molecule-1 (ICAM-1) on the endothelium of blood vessels in response to pro-inflammatory stimuli is of major importance for the regulation of local inflammation in cardiovascular diseases such as atherosclerosis, myocardial infarction and stroke. In vivo molecular imaging of ICAM-1 will improve diagnosis and follow-up of patients by non-invasive monitoring of the progression of inflammation. Results: A paramagnetic liposomal contrast agent functionalized with anti-ICAM-1 antibodies for multimodal magnetic resonance imaging (MRI) and fluorescence imaging of endothelial ICAM-1 expression is presented. The ICAM-1-targeted liposomes were extensively characterized in terms of size, morphology, relaxivity and the ability for binding to ICAM-1-expressing endothelial cells in vitro. ICAM-1-targeted liposomes exhibited strong binding to endothelial cells that depended on both the ICAM-1 expression level and the concentration of liposomes. The liposomes had a high longitudinal and transversal relaxivity, which enabled differentiation between basal and upregulated levels of ICAM-1 expression by MRI. The liposome affinity for ICAM-1 was preserved in the competing presence of leukocytes and under physiological flow conditions. Conclusion: This liposomal contrast agent displays great potential for in vivo MRI of inflammation-related ICAM-1 expression.
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