期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 6, 期 36, 页码 5775-5780出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8tb01271g
关键词
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资金
- National Natural Science Foundation of China [81771929]
- Science and Technology Program of Suzhou [SYG201605, SS201662]
- Natural Science Foundation of Tianjin [17JCQNJC13900]
- Science and Education Program of Health and Family Planning Commission of Suzhou [GWZX201504]
This study presents a facile synthesis method for the preparation of positively charged N,S co-doped carbon nanodots with excellent optical properties, and it develops a selective method for fluorescent detection of living cancer cells. The specific recognition is due to the application of an aptamer sequence, which shows high affinity and specificity to target cells. The aptamer is firstly labeled with BHQ and wraps around the carbon nanodots, then it finally quenches the fluorescence emission of the carbon nanodots. For the sensitive and selective analysis of target cells, the cells are simply mixed with the carbon nanodot-aptamer nanoconjugates, which are then centrifuged at a low speed. The recognition reaction between aptamer and target cells releases the quencher from the surface of the carbon nanodots and the centrifugation process enables the recovery of fluorescence intensity of the suspension, which reflects the level of initial cancer cells. The developed method is simple, highly selective and cost-effective, thus, it may be further exploited in clinical applications in the future.
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