期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 2, 期 39, 页码 6830-6838出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4tb00785a
关键词
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资金
- Korea Healthcare Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea [A084589]
- National Research Foundation of Korea - Ministry of Education, Science, and Technology (MEST) [NRF-2012R1A2A2A01017435]
- Korea Health Promotion Institute [HI12C0851020014] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [22A20130000065, 2013H1A2A1034262, 2012R1A2A2A01017435] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Biomedical scaffolds must be mechanically stable and highly porous three-dimensional (3D) structures to allow efficient cell-to-cell and cell-to-substrate interactions, induce blood vessel formation, and transfer oxygen, nutrients, and metabolic waste. A 3D cell-laden hybrid scaffold consisting of a combination of structural synthetic polymers and a cell-laden hydrogel is an outstanding biomedical scaffold due to its controllable mechanical properties, multiple cell loading, and homogeneous cell-distribution within the scaffold. But although this hybrid scaffold is better than conventional scaffolds, some issues must still be overcome. One is the controllability of cell release from the cell-embedded hydrogel.. Here, we propose a method to solve this problem using a geometric cell-laden hydrogel. Various cylindrical cell-laden strut sizes (diameter: 100, 200, 400, and 800 mu m) using osteoblast-like-cells (MG63) were investigated A diameter of 200 mu m was the most attractive to efficiently induce cell release and proliferation based on cell viability and fluorescence analyses. In addition, cell-laden alginate struts (200 and 800 mu m) were used to fabricate poly(epsilon-caprolactone) hybrid scaffolds; the hybrid scaffolds were intertayered with a cell-laden hydrogel (200 mu m), demonstrating significantly high osteogenic expression compared to scaffolds laden with 800 mu m struts.
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