期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 2, 期 44, 页码 7756-7764出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4tb01264j
关键词
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资金
- National Key Basic Research Program of the PRC [2014CB744504, 2011CB707700]
- Major International (Regional) Joint Research Program of China [81120108013]
- National Natural Science Foundation of China [81201175, 81371611, 81401469]
- Natural Science Foundation of Jiangsu Province [BK20130863]
- National Science Foundation for Postdoctoral Scientists of China [2013T60939, 2012M521934]
Small interfering RNA (siRNA) has great potential for gene therapy of breast cancer. The development of multifunctional nanocarriers possessing the targeted ability and multimodal imaging function is important to increase siRNA transfection efficiency and to visualize the delivery events. Herein, we demonstrate flavin mononucleotide (FMN) and polyethylenimine (PEI) modified Fe3O4@mTiO(2) nanoparticles (Fe3O4@mTiO(2)/FMN-PEI) as novel magnetic fluorescent siRNA delivery systems. The system has magnetic targeting, magnetic resonance imaging (MRI), optical imaging, and high siRNA binding ability. Survivin-siRNA delivered by this system can effectively enter into MCF-7 cells, which can be further enhanced by exerting an external magnetic field. More importantly, the siRNA delivery events including the location, trafficking, distribution, and release, can be visualized by MRI and optical imaging. Gene silencing by survivin-siRNA can cause significant protein knockdown (96%) and considerable cell apoptosis (30%), indicating the usefulness of Fe3O4@mTiO(2)/FMN-PEI as a siRNA delivery system.
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