期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 1, 期 7, 页码 1044-1051出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2tb00149g
关键词
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资金
- National Basic Research Program of China [2010CB732403]
- NSFC [41076059, 20928005, 21175025]
- Key Foundation of Education Department of Fujian Province [JK2009016]
- Natural Sciences Foundation of Fujian Province [2011J01035]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
- National Key Technologies R&D Program of China during the 12th Five-Year Plan Period [2012BAD29B06]
- Major Project of Fujian Province [2011N5008]
A kind of surface glycoprotein imprinting over magnetic Fe3O4@Au multifunctional nanofibers (NFs) was developed and investigated. Magnetic Fe3O4@Au nanoparticles (NPs) as the core materials were modified consecutively with aniline, 3-aminophenylboronic acid (APBA) and acrylic acid to introduce boronic acids and polymerizable double bonds. With horseradish peroxidase (HRP) as a glycoprotein template, thin protein-imprinted films were fabricated via radical induced graft copolymerization of monomers on the surface of the multifunctional NFs. Experimental results show that the magnetic multifunctional Fe3O4@Au NFs can not only direct the selective occurrence of imprinting polymerization, but also drive glycoprotein templates into the polymer through reversible covalent complex formation. The results show that the imprinted NFs reached saturated adsorption at 0.3 mg mL(-1) within 90 min and exhibited significant specific recognition towards the template protein. Moreover, the molecularly imprinted polymers (MIPs) were used to electrochemically detect HRP with good linearity in the range of low concentrations from 0.01 to 0.30 mg mL(-1) through molecular recognition of K-3[Fe(CN)(6)] current response. The detection limit of this method was found to be 0.005 mg mL(-1) (S/N = 3). The synthetic strategy paves the way for preparation of functional nanomaterials by molecular imprinting technique and direct detection of proteins in a more convenient, simpler and cheaper way.
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