期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 1, 期 17, 页码 2305-2314出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3tb00151b
关键词
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资金
- NRF-Technion grant [R-398-001-065-592]
- Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore
Myocardial tissue engineering offers a novel technology to improve or regenerate cardiac functions using a combination of cells, biomaterials and engineering strategies. Inspired by low-resistance pathways for electrical signal propagation in the native heart tissue, electrically conductive nanofibrous scaffolds composed of melanin, poly(L-lactide-co-epsilon-caprolactone) and gelatin were fabricated to provide electrophysiological cues to cardiac myocytes and mimic the native myocardial environment. Our results show that by increasing the concentration of melanin to 40% within the composite, the fiber diameters reduced to 153 +/- 30 nm, modulus decreased to 7.1 +/- 0.6 MPa, and conductance increased to 259.51 +/- 187.60 mu S cm(-1). Results of cell proliferation and immunostaining analysis of human cardiac myocytes demonstrated that the conductive nanofibers containing 10% melanin promote cell interaction with expression of cardiac-specific proteins compared to other scaffolds. Electrical stimulation through the scaffolds showed enhanced cell proliferation and the expression of connexin-43, signifying the potential of using melanin containing nanofibers as a suitable cardiac patch for the regeneration of infarct myocardium.
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