期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 1, 期 32, 页码 3992-3998出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3tb00519d
关键词
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资金
- National Science Foundation of China [60708024, 60877024, 61177033, 61275182]
- Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20070286058, 20090092110015]
- Science Foundation for the Excellent Youth Scholars of Southeast University
- Fundamental Research Funds for the Central University
We have developed a highly sensitive SERS-based sandwich immunoassay by utilizing two derivatives of gold nanostars simultaneously. One is densely packed self-assembled substrates of gold nanostars and the other is labeled immune nanostar aggregates. Taking advantage of the electromagnetic field coupling between tips of adjacent individual nanostars, the self-assembled substrate of gold nanostars exhibited a better SERS performance than that of gold nanoparticles. On the other hand, the immuno-aggregates made of gold stars also showed an improved SERS activity compared to those made of gold nanoparticles. Thus, by combining the self-assembled substrates of gold nanostars and immuno-aggregates of gold nanostars, highly sensitive sandwich immunoassays were obtained. The experimental results show that there existed a linear correlation between the concentration of antigens and the prominent peak intensity of SERS signals. The limit of detection (LOD) is similar to 10 fg mL(-1), 5 to 6 orders smaller than that of the conventional ELISA protocols. Such a structure will have potential wide-range applications in biological sensing and quantitative biochemical analysis.
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