期刊
JOURNAL OF CANCER
卷 9, 期 18, 页码 3382-3393出版社
IVYSPRING INT PUBL
DOI: 10.7150/jca.25136
关键词
Compound fuling granule; Mitochondrion; Ovarian cancer; Galactose metabolism; Fatty acid metabolism
类别
资金
- Program for the Cultivation of Youth talents in China Association of Chinese Medicine [QNRC2-C08]
- Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents [2015-43]
- Zhejiang Provincial Program for the Cultivation of the Young and Middle-Aged Academic Leaders in Colleges and Universities [2017-248]
- Zhejiang Provincial Project for the key discipline of traditional Chinese Medicine [2017-XK-A09]
- China Postdoctoral Science Foundation Grant [2015M581292, 2018M630694]
Our previous studies have demonstrated that the compound fuling granule (CFG), a traditional Chinese medicine, suppresses ovarian cancer cell growth, migration and metastasis. However, the underlying mechanisms remain to be fully elucidated. In this study, we found that CFG could induce mitochondrial fragmentation, mitochondrial membrane potential reduction and cytochrome c release in ovarian SKOV3 cancer cells. In addition, both metabolomics and transcriptomics approaches were applied to illustrate the systemic mechanism of CFG on ovarian cancer formation and progression. To this end, we established two tumor-bearing mice models with subcutaneous injection or tail intravenous injection. Functionally, administration of CFG suppresses in situ tumor growth and distant lung metastasis. Subsequently, gas chromatography-mass spectrometry (GC-MS) was applied to determine the metabolic alterations among the plasma samples from these in vivo models. In the subcutaneous injection model, 26 distinguishable metabolites were identified and 12 metabolic pathways were reprogrammed. Meanwhile, 19 metabolites involved in 7 metabolic pathways showed significant differences in the tail intravenous injection model. Importantly, integrative metabolomics and transcriptomics analysis showed these metabolites were highly associated with galactose metabolism and fatty acid metabolism. This study suggests that CFG may suppress ovarian cancer cell proliferation and metastasis by regulating mitochondrion-related energy metabolisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据