期刊
GENOME BIOLOGY
卷 14, 期 2, 页码 -出版社
BMC
DOI: 10.1186/gb-2013-14-2-r14
关键词
-
资金
- University of Edinburgh Staff Scholarship
- Higher Education Funding Council (Wales)
- ERC [ERC-2009-AdG-20090506]
- Medical Research Council, UK
- Wellcome Trust Senior Research Fellowship [076616, 095209]
- Wellcome Trust Centre for Cell Biology [077707]
- MRC [MC_PC_U127527202] Funding Source: UKRI
- Medical Research Council [MC_PC_U127527202] Funding Source: researchfish
Background: Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells have yet to be widely identified. Results: To search for such proteins, 23 nuclear envelope transmembrane proteins were screened for their ability to promote peripheral localization of human chromosomes in HT1080 fibroblasts. Five of these proteins had strong effects on chromosome 5, but individual proteins affected different subsets of chromosomes. The repositioning effects were reversible and the proteins with effects all exhibited highly tissue-restricted patterns of expression. Depletion of two nuclear envelope transmembrane proteins that were preferentially expressed in liver each reduced the normal peripheral positioning of chromosome 5 in liver cells. Conclusions: The discovery of nuclear envelope transmembrane proteins that can modulate chromosome position and have restricted patterns of expression may enable dissection of the functional relevance of tissue-specific patterns of radial chromosome positioning.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据