期刊
GENOME BIOLOGY
卷 12, 期 8, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/gb-2011-12-8-R73
关键词
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资金
- National Institute of Allergy and Infectious Diseases National Institutes of Health, Department of Health and Human Services [HHSN27220090018C]
- Bill and Melinda Gates Foundation [49764]
- National Human Genome Research Institute [HG03067-05]
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [U54HG003067] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [T32AI007638] Funding Source: NIH RePORTER
We have adapted a solution hybrid selection protocol to enrich pathogen DNA in clinical samples dominated by human genetic material. Using mock mixtures of human and Plasmodium falciparum malaria parasite DNA as well as clinical samples from infected patients, we demonstrate an average of approximately 40-fold enrichment of parasite DNA after hybrid selection. This approach will enable efficient genome sequencing of pathogens from clinical samples, as well as sequencing of endosymbiotic organisms such as Wolbachia that live inside diverse metazoan phyla.
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