期刊
FRONTIERS IN PLANT SCIENCE
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2018.01284
关键词
postharvest freshness; calcium ion/calmodulin; Ca2+-ATPase; gene expression; calcium signaling
资金
- National Natural Science Foundation of China [31860568, 31560563, 31160398]
- Research Fund of Higher Education of Gansu, China [2018C-14]
- Post-Doctoral Foundation of China [20100470887, 2012T50828]
- Natural Science Foundation of Gansu Province, China [1606RJZA073, 1606RJZA077, 1606RJYA252]
- Scientific Research Foundation for the Yong Graduate Supervisor of Gansu Agricultural University in Lanzhou, China [GAU-QNDS-201709]
- Feitian and Fuxi Excellent Talents in Gansu Agricultural University
Both nitric oxide (NO) and calcium ion (Ca2+)/calmodulin (CaM) have been shown to regulate the senescence of cut flowers. However, not much is known about the crosstalk between NO and Ca2+/CaM during the senescence of cut flowers. In this study, cut Oriental x Trumpet hybrid lily Manissa were used to investigate the roles and relationship between NO and Ca2+/CaM during postharvest freshness. The results show that the effects of CaCl2 or NO donor SNAP on the vase life, maximum flower diameter and hours until full opening were dose-dependent, with an optimum concentration of 20 mM CaCl2 or 100 mu M SNAP. However, Ca2+ chelators EGTA or BAPTA/AM, Ca2+ channel inhibitors LaCl3 or nifedipine and CaM antagonists W-7 or TFP inhibited the promotion of SNAP. SNAP applied alone significantly increased the endogenous Ca2+/CaM contents in cut lily flowers, while EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP decreased the advancement of SNAP. In addition, the SNAP-induced Ca2+-ATPase activity was more than twice as much as the control, but EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP also reversed the enhancement. Moreover, EGTA, BAPTA/AM, LaCl3, nifedipine, W-7, and TFP prevented the SNAP-induced upregulation of gene expression of CaM, CBL1, and CBL3, which is associated with calcium signaling pathway. Overall, these results suggest that Ca2+/CaM may function as downstream molecules in NO-regulated senescence of cut flowers.
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