期刊
FRONTIERS IN MICROBIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2018.01695
关键词
schistosomiasis japonica; diagnosis; gene polymorphism; SISP-13; biomarker
类别
资金
- National Natural Science Foundation of China [81601782, 81430051]
- Natural Science Foundation of Shanghai [15ZR1443500]
Schistosomiasis japonica is one of the most prevalent parasitic diseases in China. The scarcity of effective diagnostic tools is a major factor that contributes to the high prevalence of schistosomiasis japonica. SjSP-13 is a promising serological diagnostic biomarker of the disease. However, it is unclear whether polymorphisms in SjSP-13 affect its diagnostic efficacy and immunogenicity. Here, we found the SjSP-13 gene was highly polymorphic, and all the alleles of the gene were clustered into two clades, Glade A and B. SjSP-13.6 and SjSP-13.25, the representative alleles of Glade A and B, were produced in Escherichia coli. The diagnostic value of SjSP-13.6 (AUC = 0.983 +/- 0.006), was found to be similar to the SjSP-13.25 (AUC = 0.973 +/- 0.009) by receiver operating characteristic (ROC) analysis. SjSP-13.6 and SjSP-13.25 have the same specificity (96.7%), while the sensitivity of SjSP-13.6 (90.4%) is slightly but not significantly higher than SjSP-13.25 (85.2%). The combination use of the two alleles (SjSP-13.6/25) didn't increase the diagnostic performance of SjSP-13 as the AUC value of SjSP-13.6/25 is 0.977 +/- 0.009, lower than individual SjSP-13.6 (AUC = 0.983 +/- 0.006). In addition, we found the immunogenicity of Glade A alleles is significantly higher than Glade B in Schistosoma japonicum naturally infected animals and patients, as the mean antibody levels of SjSP-13.6 was significantly higher than SjSP-13.25. We conclude that polymorphisms of the SjSP-13 gene should not affect its diagnostic efficacy, and it is not necessary to combine the alleles of the two clades for diagnosis of schistosomiasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据