期刊
FRONTIERS IN MICROBIOLOGY
卷 5, 期 -, 页码 -出版社
FRONTIERS RESEARCH FOUNDATION
DOI: 10.3389/fmicb.2014.00403
关键词
slippage; primer-template misalignment; DNA polymerases; strand displacement activity; Archaea
类别
资金
- Ministerio de Educacion y Ciencia [BFU2007-64153]
- Junta de Andalucia [P09-CVI-5428]
- National Research Agency [ANR-10-JCJC-1501 01]
- EMBO
- FPI predoctoral Fellowship from Ministerio de Educacion y Ciencia, Spain [BES-2005-10150]
Replication slippage or slipped-strand mispairing involves the misalignment of DNA strands during the replication of repeated DNA sequences, and can lead to genetic rearrangements such as microsatellite instability. Here, we show that PolB and PolD replicative DNA polymerases from the archaeal model Pyrococcus abyssi (Pab) slip in vitro during replication of a single-stranded DNA template carrying a hairpin structure and short direct repeats. We find that this occurs in both their wild-type (exo+) and exonuclease deficient (exo-) forms. The slippage behavior of PabPolB and PabPolD, probably due to limited strand displacement activity, resembles that observed for the high fidelity P furiosus (Pfu) DNA polymerase. The presence of PabPCNA inhibited PabPolB and PabPolD slippage. We propose a model whereby PabPCNA stimulates strand displacement activity and polymerase progression through the hairpin, thus permitting the error-free replication of repetitive sequences.
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