期刊
FRONTIERS IN MICROBIOLOGY
卷 5, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2014.00547
关键词
horizontal gene transfer; tetR; repressor; oxidative stress; EMSA; proteome profiling
类别
资金
- Special Fund for Agro-scientific Research of China [201303015]
- National Natural Science Foundation of China [31200003]
- Scholarship Award for Excellent Doctoral Student granted by Ministry of Education, 12th Five Years Key Programs for Science and Technology Development of China [2012BAK11B02, 2012BAK11B06]
- Zhejiang Provincial Nature Science Foundation of China [LY12C14007]
- China Post-doctoral Special Foundation [317000-X91206]
Acidovorax avenae subsp. avenae is the causal agent of bacterial brown stripe disease in rice. In this study, we characterized a novel horizontal transfer of a gene cluster, including tetR, on the chromosome of A. avenae subsp. avenae RS-1 by genome-wide analysis. TetR acted as a repressor in this gene cluster and the oxidative stress resistance was enhanced in tetR-deletion mutant strain. Electrophoretic mobility shift assay demonstrated thatTetR regulator bound directly to the promoter of this gene cluster. Consistently, the results of quantitative real-time PCR also showed alterations in expression of associated genes. Moreover, the proteins affected byTetR under oxidative stress were revealed by comparing proteomic profiles of wild-type and mutant strains via 1D SDS-PAGE and LC-MS/MS analyses. Taken together, our results demonstrated that tetR gene in this novel gene cluster contributed to cell survival under oxidative stress, and TetR protein played an important regulatory role in growth kinetics, biofilm-forming capability, superoxide dismutase and catalase activity, and oxide detoxicating ability.
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