期刊
FRONTIERS OF EARTH SCIENCE
卷 6, 期 4, 页码 383-391出版社
SPRINGER
DOI: 10.1007/s11707-012-0336-9
关键词
anammox; n-damo; co-occurrence; saline lakes; Qinghai-Tibetan Plateau
资金
- National Natural Science Foundation of China [41002123, 41030211, 41121001]
- National Basic Research Program of China [2011CB808800]
- Fundamental Research Founds for National University
- China University of Geosciences (Wuhan)
- CAS [A0961]
Nitrite-dependent anaerobic methane-oxidizing (n-damo) bacteria and anaerobic ammonia oxidizing (anammox) bacteria are two groups of microorganisms involved in global carbon and nitrogen cycling. In order to test whether the n-damo and anammox bacteria co-occur in natural saline environments, the DNA and cDNA samples obtained from the surficial sediments of two saline lakes (with salinity of 32 and 84 g/L, respectively) on the Tibetan Plateau were PCR-amplified with the use of anammox- and n-damo-specific primer sets, followed by clone library construction and phylogenetic analysis. DNA and cDNA-based clones affiliated with n-damo and anammox bacteria were successfully retrieved from the two samples, indicating that these two groups of bacteria can co-occur in natural saline environments with salinity as high as 84 g/L. Our finding has great implications for our understanding of the global carbon and nitrogen cycle in nature.
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