期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 4, 期 -, 页码 -出版社
FRONTIERS RESEARCH FOUNDATION
DOI: 10.3389/fcimb.2014.00094
关键词
oral microbiology; interspecies interaction; quorum sensing
资金
- Universitas Indonesia (RUUI), Indonesia
- Division for Earth and Life Sciences (ALW)
- Netherlands Organization for Scientific Research (NWO)
- Young Investigator
- Human Frontier Science Program (HFSP) [RGY0072/2007]
- University of Amsterdam
Aggregatibacter actinomycetemcomitans, a Gram-negative bacterium, and Candida albicans, a polymorphic fungus, are both commensals of the oral cavity but both are opportunistic pathogens that can cause oral diseases. A. actinomycetemcomitans produces a quorum-sensing molecule called autoinducer-2 (Al-2), synthesized by LuxS, that plays an important role in expression of virulence factors, in intra- but also in interspecies communication. The aim of this study was to investigate the role of Al-2 based signaling in the interactions between C. albicans and A. actinomycetemcomitans. A. actinomycetemcomitans adhered to C. albicans and inhibited biofilm formation by means of a molecule that was secreted during growth. C. albicans biofilm formation increased significantly when co-cultured with A. acnnomycetemcomitans luxS, lacking Al-2 production. Addition of wild-type-derived spent medium or synthetic Al-2 to spent medium of the luxS strain, restored inhibition of C. albicans biofilm formation to wild-type levels. Addition of synthetic Al-2 significantly inhibited hypha formation of C. albicans possibly explaining the inhibition of biofilm formation. Al-2 of A. actinomycetemcomitans is synthesized by LuxS, accumulates during growth and inhibits C. albicans hypha- and biofilm formation. Identifying the molecular mechanisms underlying the interaction between bacteria and fungi may provide important insight into the balance within complex oral microbial communities.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据