期刊
AMB EXPRESS
卷 5, 期 -, 页码 -出版社
SPRINGER
DOI: 10.1186/s13568-015-0112-9
关键词
Flavoprotein; Monooxygenase; Oligomerization; FAD binding; Rhodococcus opacus 1CP; Styrene epoxidation
资金
- European Social Fund and the Saxonian Government [GETGEOWEB: 100101363]
Styrene monooxygenases (SMOs) are flavoenzymes catalyzing the epoxidation of styrene into styrene oxide. SMOs are composed of a monooxygenase (StyA) and a reductase (StyB). The latter delivers reduced FAD to StyA on the expense of NADH. We identified Rhodococcus opacus 1CP as the first microorganism to possess three different StyA isoforms occurring in two systems StyA1/StyA2B and StyA/StyB, respectively. The hydrodynamic properties of StyA isozymes were found to be modulated by the binding of the (reduced) FAD cofactor. StyA1 and SyA2B mainly occur as dimers in their active forms while StyA is a monomer. StyA1 showed the highest epoxidation activity and excellent enantioselectivity in aromatic sulfoxidation. The hydrodynamic and biocatalytic properties of SMOs from strain 1CP are of relevance for investigation of possible industrial applications.
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