期刊
ELIFE
卷 3, 期 -, 页码 -出版社
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.03735
关键词
Translation; decoding; codon bias; codon usage; synonymous codons
类别
资金
- NIH grant [R01 GM098400]
- NSF grants [DBI-1060572, IIS-1017181]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1355990] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1060572] Funding Source: National Science Foundation
Most amino acids can be encoded by several synonymous codons, which are used at unequal frequencies. The significance of unequal codon usage remains unclear. One hypothesis is that frequent codons are translated relatively rapidly. However, there is little direct, in vivo evidence regarding codon-specific translation rates. Here, we generate high-coverage data using ribosome profiling in yeast, analyze using a novel algorithm, and deduce events at the A and P-sites of the ribosome. Different codons are decoded at different rates in the A-site. In general frequent codons are decoded more quickly than rare codons, and AT-rich codons are decoded more quickly than GC-rich codons. At the P-site, proline is slow in forming peptide bonds. We also apply our algorithm to short footprints from a different conformation of the ribosome, and find strong, amino-acid specific (not codon-specific) effects that may reflect interactions with the exit tunnel of the ribosome.
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