期刊
ELIFE
卷 3, 期 -, 页码 -出版社
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.03058
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资金
- European Research Council [DARCGENS 249869]
- Medical Research Council
- Max-Planck-Gesellschaft
- European Research Council
- Swiss National Science Foundation
- Wellcome Trust
- Austrian Academy of Sciences
- Austrian Science Fund [FWF F4302-B09]
- European Molecular Biology Laboratory
- Cancer Research UK
- National Human Genome Research Institute [U54 HG007004-2]
- BBSRC [BBS/E/T/000PR5885, BBS/E/T/000PR6193] Funding Source: UKRI
- MRC [MC_UU_12009/4, MC_UU_12021/1, MC_U137761446, G1000801] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/T/000PR5885, BBS/E/T/000PR6193] Funding Source: researchfish
- Cancer Research UK [11832] Funding Source: researchfish
- Medical Research Council [MC_U137761446, G1000801, G1000801b, MC_UU_12021/1, MC_UU_12009/4] Funding Source: researchfish
- Wellcome Trust [095606/Z/11/Z] Funding Source: researchfish
Although a small number of the vast array of animal long non-coding RNAs (IncRNAs) have known effects on cellular processes examined in vitro, the extent of their contributions to normal cell processes throughout development, differentiation and disease for the most part remains less clear. Phenotypes arising from deletion of an entire genomic locus cannot be unequivocally attributed either to the loss of the IncRNA per se or to the associated loss of other overlapping DNA regulatory elements. The distinction between cis- or trans-effects is also often problematic. We discuss the advantages and challenges associated with the current techniques for studying the in vivo function of IncRNAs in the light of different models of IncRNA molecular mechanism, and reflect on the design of experiments to mutate IncRNA loci. These considerations should assist in the further investigation of these transcriptional products of the genome.
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