期刊
ADVANCED SCIENCE
卷 3, 期 2, 页码 -出版社
WILEY
DOI: 10.1002/advs.201500155
关键词
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资金
- National Basic Research Program of China [2012CB933301]
- National Natural Science Foundation of China [21171098, 51473078]
- Program for New Century Excellent Talents in University [NCET-12-0740]
- Ministry of Education of China [20133223110006]
- Natural Science Foundation of Jiangsu Province of China [BK20130038, BM2012010]
- Synergetic Innovation Center for Organic Electronics and Information Displays
- Priority Academic Program Development of Jiangsu Higher Education Institutions [YX03001]
Molecular oxygen (O-2) plays a key role in many physiological processes, and becomes a toxicant to kill cells when excited to O-1(2). Intracellular O-2 levels, or the degree of hypoxia, are always viewed as an indicator of cancers. Due to the highly efficient cancer therapy ability and low side effect, photodynamic therapy (PDT) becomes one of the most promising treatments for cancers. Herein, an early-stage diagnosis and therapy system is reported based on the phosphorescent conjugated polymer dots (Pdots) containing Pt(II) porphyrin as an oxygen-responsive phosphorescent group and O-1(2) photosensitizer. Intracellular hypoxia detection has been investigated. Results show that cells treated with Pdots display longer lifetimes under hypoxic conditions, and time-resolved luminescence images exhibit a higher signal-to-noise ratio after gating off the short-lived background fluorescence. Quantification of O-2 is realized by the ratiometric emission intensity of phosphorescence/fluorescence and the lifetime of phosphorescence. Additionally, the PDT efficiency of Pdots is estimated by flow cytometry, MTT cell viability assay, and in situ imaging of PDT induced cell death. Interestingly, Pdots exhibit a high PDT efficiency and would be promising in clinical applications.
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