期刊
ADVANCED SCIENCE
卷 2, 期 3, 页码 -出版社
WILEY-BLACKWELL
DOI: 10.1002/advs.201400009
关键词
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资金
- National Basic Research Program of China [2012CB933301]
- National Natural Science Foundation of China [21204038, 81273409, 61205195, 51103074, 51173081]
- Ministry of Education of China [IRT1148, 20123223110007, 20123223120011]
- National Synergistic Innovation Center for Advanced Materials (SICAM)
- Sci-tech Support Plan of Jiangsu Province [BE2014719]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Hybridbio/-synthetic sensory conjugated polymer nanoparticles (CPNs) are developed for selective label-free detection of target ssDNA in serum. Carboxylic acid-functionalized anionic polyfluorene nanoparticles are rationally designed as signal amplifying unit to bioconjugate with amine functionalized single stranded oligonucleotides as a receptor. The covalent DNA coating can significantly improve the photostability of the DNA-bioconjugated CPNs over a wide range of buffer conditions. Better ssDNA discrimination for the DNA-bioconjugated CPNs sensor is achieved owing to increased interchain interactions and more efficient exciton transport in nanoparticles. The distinguishable fluorescent color for DNA-bioconjugated CPNs in the presence of target ssDNA allows naked-eye detection of ssDNA under UV irradiation.
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