期刊
ELIFE
卷 1, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.00184
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资金
- Howard Hughes Medical Institute [012229]
- Welch Foundation [I-1578]
- Natural Science Foundation of Zhejiang Province, China [R2100439]
- National Institutes of Health [RO1 GM071621]
- David and Lucile Packard Foundation [2004-27671]
- Specialized Research Fund for the Doctoral Program of Higher Education, China [20110101110122]
The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K+ channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K+ channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD(+) activate the GsuK channel, whereas Ca2+ serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K+ channels.
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