期刊
CHEMISTRYOPEN
卷 2, 期 3, 页码 93-98出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/open.201300003
关键词
electrochemiluminescence; quantum dots; signal amplification; TiO2 nanotubes
资金
- National Natural Science Foundation of China [21005012, 21275026, 11004025, 11174046, 21005016]
- New Century Excellent Talents in the University of China [NCET-10-0305]
- Fundamental Research Funds for the Central Universities [N110805001, N110705002]
- Deutsche Forschungsgemeinschaft (DFG)
- DFG cluster of excellence Engineering of Advanced Materials (EAM)
Self-organized TiO2-nanotube layers can be used for immunoassay-type sensing in combination with amplifying CdTe labels in a direct and very sensitive electrochemiluminescent (ECL) configuration. Key properties for this method are the conductivity of the TiO2 nanotubes, and their transparency for light emitted from the CdTe labels at approximately 2.4 eV. To demonstrate the potential of this platform, we constructed a sandwich-type immunoassay onto the TiO2-nanotube wall with a layer of (3-aminopropyl)triethoxysilane as the cross-linker for antibody immobilization. For the counter part of the sandwich, we created an amplification system consisting of TiO2 nanobeads carrying the secondary antibody and multiple CdTe quantum dots (multiQD). For antigen (IgG) detection, we find that this combination of 3D transparent electrode with multiQD labels allows for an ECL detection limit of 0.05 pg mL(-1) and a linearity of the signal in the range of 0.1-10(8) pg mL(-1).
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