期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 71, 期 -, 页码 876-888出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2053230X15008766
关键词
nuclear transport; Mex67; Chaetomium thermophilum; RNA binding
资金
- Medical Research Council [U105178939]
- Wellcome Trust Programme grant
- MRC [MC_U105178939] Funding Source: UKRI
- Medical Research Council [MC_U105178939] Funding Source: researchfish
Members of the Mex67-Mtr2/NXF-NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67-Mtr2 that facilitate mRNA nuclear export.
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