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Coexpression of double or triple copies of the rabies virus glycoprotein gene using a 'self-cleaving' 2A peptide-based replication-defective human adenovirus serotype 5 vector
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Virus-free induction of pluripotency and subsequent excision of reprogramming factors
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piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
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Reprogramming of murine and human somatic cells using a single polycistronic vector
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Induced Pluripotent Stem Cell Generation Using a Single Lentiviral Stem Cell Cassette
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Use of the viral 2A peptide for bicistronic expression in transgenic mice
Georgios Trichas et al.
BMC BIOLOGY (2008)
Local secretion of anti-CTLA-4 enhances the therapeutic efficacy of a cancer immunotherapy with reduced evidence of systemic autoimmunity
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Retroviral Bicistronic Vectors
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Retroviral transfer of a dominant TCR prevents surface expression of a large proportion of the endogenous TCR repertoire in human T cells
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Development of optimal bicistronic lentiviral vectors facilitates high-level TCR gene expression and robust tumor cell recognition
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GENE THERAPY (2008)
Expression of heterologous genes in oncolytic adenoviruses using picornaviral 2A sequences that trigger ribosome skipping
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Scalable signaling mediated by T cell antigen receptor-CD3 ITAMs ensures effective negative selection and prevents autoimmunity
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Marking Embryonic Stem Cells with a 2A Self-Cleaving Peptide: A NKX2-5 Emerald GFP BAC Reporter
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Splicing mediates the activity of four putative cellular internal ribosome entry sites
Brian T. Baranick et al.
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Lentiviral-Mediated HoxB4 Expression in Human Embryonic Stem Cells Initiates Early Hematopoiesis in a Dose-Dependent Manner but Does Not Promote Myeloid Differentiation
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Viral 2A peptides allow expression of multiple proteins from a single ORF in Transgenic zebrafish embryos
Elayne Provost et al.
GENESIS (2007)
Stable gene transfer and expression in human primary T cells by the Sleeping Beauty transposon system
X Huang et al.
BLOOD (2006)
Generation of T-cell receptor retrogenic mice
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FMDV-2A sequence and protein arrangement contribute to functionality of CYP2B1-reporter fusion protein
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ANALYTICAL BIOCHEMISTRY (2005)
Development of 2A peptide-based strategies in the design of multicistronic vectors
AL Szymczak et al.
EXPERT OPINION ON BIOLOGICAL THERAPY (2005)
Stable antibody expression at therapeutic levels using the 2A peptide
JM Fang et al.
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A second look at cellular mRNA sequences said to function as internal ribosome entry sites
M Kozak
NUCLEIC ACIDS RESEARCH (2005)
Targeting of proteins derived from self-processing polyproteins containing multiple signal sequences
P de Felipe et al.
TRAFFIC (2004)
Enhanced in vivo selection of bone marrow cells by retroviral-mediated coexpression of mutant O6-methylguanine-DNA-methyltransferase and HOXB4
MD Milsom et al.
MOLECULAR THERAPY (2004)
Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector
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Co-translational, intraribosomal cleavage of polypeptides by the foot-and-mouth disease virus 2A peptide
P de Felipe et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2003)
Expressing multiple genes in a single open reading frame with the 2A region of foot-and-mouth disease virus as a linker
CL Ma et al.
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Recombinant AAV vectors containing the foot and mouth disease virus 2A sequence confer efficient bicistronic gene expression in cultured cells and rat substantia nigra neurons
S Furler et al.
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Retroviral vector-mediated expression of HoxB4 in hematopoietic cells using a novel coexpression strategy
H Klump et al.
GENE THERAPY (2001)
Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'
MLL Donnelly et al.
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The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences
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