期刊
BIOMED RESEARCH INTERNATIONAL
卷 2013, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2013/963457
关键词
-
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [79-155]
In the present investigation, we examined the effect of Hyuganatsu (Citrus tamurana) extract (HE) on skin fibroblast (TIG-119) proliferation and migration during in vitro wound healing. HE selectively inhibited proliferation of TIG-119 cells at higher concentration (> 1.0mg/mL); at lower concentrations (0.1, 0.25, 0.5, and 0.75mg/mL), it exhibited linear and time-dependent cell proliferation. In vitro scratchwound healing studies showed that the HE also accelerated the migration of cells towards the wounded region. Cytometric analysis demonstrated that HE extract did not alter G(1)/0 and S phases of cell cycle in any concentration studied; however, G(2)/M phases of cell cycle were significantly (P < 0.05) accelerated at 0.75mg/mL dose. RT-PCR and Western blotting analysis indicated that HE markedly overexpressed levels of Rac-1, Rho-A, and Cdc-42 mRNA and the respective proteins. Cyclin-dependent kinases (Cdk-1 and -2) gene expression activity was significantly (P < 0.05) increased, but protein content decreased during treatment with HE. The induction of Cdk-1 and -2 by HE was abolished by inhibitors, transcription (DRB), and translation (CHX), implying transcriptional regulation that required de novo protein synthesis.
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