期刊
BIOMED RESEARCH INTERNATIONAL
卷 2013, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2013/694269
关键词
-
资金
- Ministry of Economy, Trade and Industry (METI) of Japan
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [23406017, 25246029] Funding Source: KAKEN
We have recently treated with N-2 gas plasma and achieved inactivation of bacteria. However, the effect of N-2 gas plasma on viruses remains unclear. With the aim of developing this technique, we analyzed the virucidal effect of N-2 gas plasma on influenza virus and its influence on the viral components. We treated influenza virus particles with inert N-2 gas plasma (1.5 kpps; kilo pulses per second) produced by a short high-voltage pulse generated from a static induction thyristor power supply. A bioassay using chicken embryonated eggs demonstrated that N-2 gas plasma inactivated influenza virus in allantoic fluid within 5 min. Immunochromatography, enzyme-linked immunosorbent assay, and Coomassie brilliant blue staining showed that N-2 gas plasma treatment of influenza A and B viruses in nasal aspirates and allantoic fluids as well as purified influenza A and B viruses induced degradation of viral proteins including nucleoprotein. Analysis using the polymerase chain reaction suggested that N-2 gas plasma treatment induced changes in the viral RNA genome. Scanning electron microscopy analysis showed that aggregation and fusion of influenza viruses were induced by N-2 gas plasma treatment. We believe these biochemical changes(m)ay contribute to the inactivation of influenza viruses by N-2 gas plasma.
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