期刊
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
卷 46, 期 -, 页码 S318-S327出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/21691401.2018.1492420
关键词
Hypoxia; oxygen nanobubbles; HIF-1 alpha; MDA-MB231
资金
- Nano-Material Technology Development Program of the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2017M3A7B8061942]
- National Research Foundation of Korea [2017M3A7B8061942] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Hypoxia, which results from an inadequate supply of oxygen, is a major cause of concern in cancer therapy as it is associated with a reduction in the effectiveness of chemotherapy and radiotherapy in cancer treatment. Overexpression and stabilization of hypoxia-inducible factor 1 alpha (HIF-1 alpha) protein in tumours, due to hypoxia, results in poor prognosis and increased patient mortality. To increase oxygen tension in hypoxic areas, micro- and nanobubbles have been investigated by various researchers. In the present research, lipid-shelled oxygen nanobubbles (ONBs) were synthesized through a sonication method to reverse hypoxic conditions created in a custom-made hypoxic chamber. Release of oxygen gas from ONBs in deoxygenated water was evaluated by measuring dissolved oxygen. Hypoxic conditions were evaluated by performing in vitro experiments on MDA-MB231 breast cancer cells through the expression of HIF-1 alpha and the fluorescence of image-iT (TM) hypoxia reagent. The results indicated the degradation of HIF-1 alpha after the introduction of ONBs. We propose that ONBs are successful in reversing hypoxia, downregulating HIF-1 alpha, and improving cellular conditions, leading to further medical applications.
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