4.5 Article

An Fe3O4-nanoparticles-based amperometric biosensor for creatine determination

期刊

出版社

TAYLOR & FRANCIS LTD
DOI: 10.3109/10731199.2012.712044

关键词

Amperometry; carbon paste; creatinase; enzyme electrode; nanoparticle; sarcosine oxidase

资金

  1. Ankara University Research Fund [11B4240005]

向作者/读者索取更多资源

An amperometric biosensor for the detection of creatine was designed, based on carbon paste electrode modified with Fe3O4 nanoparticles. Electron transfer properties of unmodified and Fe3O4-nanoparticles-modified carbon paste electrodes were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. Fe3O4 nanoparticles increased the surface area and electric conductivity of the electrode, thus enhancing the sensitivity of the electrode. Optimum pH, buffer concentration, working potential and enzyme loading were selected as 7.0; 0.05 mol L-1; + 0.30 V and 2.0 Unit creatinase (CI), 1.0 Unit sarcosine oxidase (SO), respectively. The purposed biosensor exhibited linear response from 2.0 x 10(-7) mol L-1 to 3.8 x 10(-6) mol L-1 and from 9.0 x 10(-6) mol L-1 to 1.2 x 10(-4) mol L-1 with a detection limit of 2.0 x 10(-7) mol L-1. Biosensor was used for determination of creatine in commercial creatine powder samples and showed a good sensing performance.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据