期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 5, 期 -, 页码 258-263出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.algal.2014.03.006
关键词
Porphyridium; Polysaccharide; B-phycoerythrin; Microalgae
资金
- French Agency for Research (ANR) [ANR-10-BLAN-0936]
- Agence Nationale de la Recherche (ANR) [ANR-10-BLAN-0936] Funding Source: Agence Nationale de la Recherche (ANR)
Porphyridium species are exploited for the production of exopolysaccharides and B-phycoerythrin, two high-value compounds. Extraction and purification of these two substances often involve antagonistic processes (precipitation with ethanol, isoelectric precipitation, aqueous two-phase systems, chromatography and ultrafiltration) that work better for one than for the other. We developed a two-step membrane process using polyethersulfone flat membranes with a filtration surface of 0.1 m(2) to recover both B-phycoerythrin and soluble polysaccharides from Porphyridium biomass with high polysaccharide content. A first ultrafiltration at 300,000 yielded high molecular weight polysaccharides. The resulting 300,000 permeate was then run through a second, 10,000 membrane to recover BPE. This procedure enabled us to extract 48% of B-phycoerythrin free of polysaccharide with a purity index of 2.3. (C) 2014 Elsevier B.V. All rights reserved.
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