期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 1, 期 1, 页码 17-21出版社
ELSEVIER
DOI: 10.1016/j.algal.2012.03.001
关键词
Induced mutagenesis; Nannochloropsis sp.; Ethyl methane sulfonate; Fluorescence activated cell sorting; SYTOX Green; Biodiesel
资金
- Agency for Science, Technology and Research of Singapore (A* STAR)
Nannochloropsis sp., a promising marine microalga for biodiesel feedstock production, was treated with the mutagen ethyl methanesulfonate (EMS) to induce random mutagenesis which was then followed by flow cytometric cell sorting to isolate mutants with an enhanced intracellular lipid content. Treatment resulted in up to a four-fold increase in total fatty acid content in the mutant strain, a 30% increase in palmitoleic acid (16:1) and a 45% decrease of eicosapentaenoic acid (20:5n3) relative to that of the wild-type (WT). The mutant produced fatty acid more prolifically than the WT during both exponential and stationary phases of growth. This study highlights the ability to enhance intracellular lipid accumulation in microalgae using random mutagenesis in conjunction with flow cytometric cell sorting. (C) 2012 Elsevier B.V. All rights reserved.
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