期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 1, 期 12, 页码 1220-1230出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.5b00445
关键词
bone tissue engineering; PCL foam; polydopamine coating; osteogenesis; mesenchymal stem cells
资金
- National Institutes of Health [R03 EB015202]
Recently, a novel shape memory polymer foam based on the photopolymerization of poly(epsilon-caprolactone) diacrylate (PCLDA) has been developed. These PCLDA foams enter a temporary softened state when briefly treated with warm saline (T-saline > T-m of PCLDA), allowing them to conform to irregular bone defect boundaries prior to shape setting. When coated with a mechanically stable polydopamine (PD) layer, these PCLDA foams have previously been demonstrated to induce hydroxyapatite deposition. In the present study, the osteoinductivity of these self-fitting PD-coated PCLDA (PD-PCLDA) materials was evaluated relative to uncoated PCLDA (U-PCLDA) controls using bone marrow-derived human mesenchymal stem cells (h-MSCs). When cultured in the absence of osteogenic media supplements, PD-PCLDA scaffolds expressed similar levels of Runx2, alkaline phosphatase, and osteopontin protein as U-PCLDA scaffolds cultured in the presence of osteogenic media supplements. In addition, PD-PCLDA scaffolds cultured without osteogenic supplements did not significantly promote undesired lineage progression (e.g., adipogenesis or chondrogenesis) of h-MSCs. Cumulatively, these data indicate that PD-PCLDA materials display increased osteoinductivity relative to U-PCLDA substrates. Future studies will examine tethered osteogenic factors or peptides toward augmenting the osteoinductive properties of the PD-PCLDA foams.
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