期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 1, 期 3, 页码 166-174出版社
AMER CHEMICAL SOC
DOI: 10.1021/ab5001063
关键词
macrophage phenotypes; cytokines; TNF-alpha; IL-I0; polymer properties
资金
- National Science Foundation [CBET 1227867]
- Roy J. Carver Charitable Trust [13-4265]
- NSF ARI-R2 [CMMI-0963224]
Of central importance to tissue engineering and drug delivery is identifying polymer parameters that increase or decrease specific cytokines in response to biomaterials. In this study, we have interrogated the effects of material descriptors and material.characteristics on pro-inflammatory, pro-angiogenic, and naive macrophages using polymeric particles (similar to 600 nm), functionalized with 13 different moieties. We characterized tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) secretion for the three macrophage populations and used the quantitative structure-activity relationship method (QSAR) to accurately predict cytokine secretion for the different macrophage phenotypes. The findings presented here demonstrate that altering cellular responses to polymers can be achieved through exploiting material parameters. For pro-inflammatory macrophages, polarity and the ability to hydrogen bond appear to significantly impact TNF-alpha secretion while charge impacted pro-angiogenic macrophages. Naive cells were impacted by charge in a similar manner as the proangiogenic cells; however, hydrophilicity also increased TNF-alpha secretion in these cells. For IL-10 secretion, hydrogen bonding was very negatively correlated with pro-inflammatory cells, whereas it was positively correlated with pro-angiogenic cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据