期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 2, 期 1, 页码 19-29出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.5b00259
关键词
bacterial cellulose; nanofibrous scaffold; hollow organ; urethral reconstruction
资金
- National Natural Science Foundation of China [51573024, 81170641, 81100488, 81370795]
- department of education for postgraduate event [20130073110021]
- Fundamental Research Funds for Central Universities [CUSF-DH-D-2015029]
In this study, we built a bilayer nanofibrous material by utilizing the gelatinization properties of potato starch (PS) to interrupt bacterial cellulose (BC) assembly during static culture to create more free spaces within the fibrous network. Then, muscle cells were cultured on the loose surface of the BC/PS scaffolds to build biomaterials for hollow organ reconstruction. Our results showed that the BC/PS scaffolds exhibited similar mechanical characters to those in the traditional BC scaffolds. And the pore sizes and porosities of BC/PS scaffolds could be controlled by adjusting the starch content. The average nanofiber diameters of unmodified BC and BC/PS composites is approximately to that of the urethral acellular matrix. Those scaffolds permit the muscle cells infiltration into the loose layer and the BC/PS membranes with muscle cells could enhance wound healing in vivo and vitro. Our study suggested that the use of bilayer BC/PS nanofibrous scaffolds may lead to improved vessel formation. BC/PS nanofibrous scaffolds with muscle cells enhanced the repair in dog urethral defect models, resulting in patent urethra. Improved organized muscle bundles and epithelial layer were observed in animals treated with BC/PS scaffold seeded by muscle cells compared with those treated with pure BC/PS scaffold. This study suggests that this biomaterial could be suitable for tissue engineered urinary tract reconstruction and this type of composite scaffold could be used for numerous other types of hollow organ tissue engineering grafts, including vascular, bladder, ureter, esophagus, and intestine.
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