4.6 Article

Self-Sufficient Flow-Biocatalysis by Coimmobilization of Pyridoxal 5′-Phosphate and ω-Transaminases onto Porous Carriers

期刊

ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 6, 期 10, 页码 13151-13159

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.8b02672

关键词

omega-Transaminase; Enzyme immobilization; Cofactor coimmobilization; Self-sufficient biocatalyst; Flow reactions

资金

  1. ARAID foundation
  2. MINECO [BIO2015-69887-R]
  3. BBSRC [BB/P002536/1]
  4. COST action [CM1303]
  5. Mexican Council for Science and Technology (CONACyT)
  6. BBSRC [BB/P002536/1] Funding Source: UKRI

向作者/读者索取更多资源

We expanded the application of self-sufficient heterogeneous biocatalysts containing coimmobilized omega-transaminases and pyridoxal 5'-phosphate (PLP) to efficiently operate packed-bed reactors in continuous flow. Using a omega-transaminase from Halomonas elongata coimmobilized with PLP onto porous methacrylate-based carriers coated with polyethylenimine, we operated a packed-bed reactor continuously for up to 50 column volumes at 1.45 mL x min(-1) in the enantioselective deamination of model amines (alpha-methylbenzyl amine), yielding >90% conversion in all cycles without exogenous addition of cofactor. In this work, we expanded the concept of self-sufficient heterogeneous biocatalysts to other w-transaminases such as the ones from Chromobacterium violaceum and Pseudomonas fluorescens. We found that enzymes with lower affinities toward PLP present lower operational stabilities in flow, even when coimmobilizing PLP. Furthermore, omega-transaminases coimmobilized with PLP were successfully implemented for the continuous synthesis of amines and the sustainable metrics were assessed. These results give some clues to exploit PLP-dependent omega-transaminases under industrially relevant continuous operations in a more cost-effective and environmentally friendly process.

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